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Is a complete on-line instructional resource. The widespread availability of fluorometers and fluorescent DNA-binding dyes makes fluorescence measurement one other well-liked choice for determining of DNA yield and focus. Fluorescence methods are more sensitive than absorbance, particularly for low-concentration samples, and the use of DNA-binding dyes allows more particular measurement of DNA than spectrophotometric methods permits. Hoechst bisbenzimidazole dyes, PicoGreen® and QuantiFluor dsDNA dyes selectively bind double-stranded DNA. The availability of single-tube and microplate fluorometers gives flexibility for studying samples in PCR tubes, cuvettes or multiwell plates and makes fluorescence measurement a handy modern various to the more conventional absorbance methods.
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